Acute kidney injury (AKI) is an acute reduction in kidney function characterised by an increase of serum creatinine and reduction in urine production, and is a known risk factor for chronic kidney disease. Reduced kidney function can have adverse effects on bone. There is a well-established relationship between chronic kidney disease and poor bone health; however, the relationship between AKI and bone health has not been well defined. A retrospective cohort study was conducted to determine the association, if any, of AKI with development of osteoporosis using de-identified data from a regional health district in News South Wales, Australia. Data included hospital admissions for adults, 18 years and older, who presented to a hospital or pathology service between 2008 and 2017. Presence of CKD was confirmed using serum estimated glomerular filtration rate in accordance with the Kidney Disease: Improving Global Outcomes classification. Presence of osteoporosis was confirmed in accordance with the 10th edition of the Australian modification of the international classification of diseases. The study included 14,590 patients with AKI. Of those patients, 13.8% developed osteoporosis (n = 2,013). Preliminary chi-square analysis indicated a significant association between AKI and osteoporosis (p < 0.001). Osteoporosis is a debilitating disease that often results in fracture and a subsequent decrease in quality-of-life. Importantly, AKI is not currently considered a risk factor for osteoporosis. It is imperative that the actual risk AKI presents for the development of osteoporosis is determined, as this may impact clinical management of patients post-AKI.